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Analyzing and Building Nucleic Acid Structures with 3DNA
Published on: April 26, 2013
Single-molecule FRET analysis of protein-DNA complexes
Mike Heilemann1, Ling Chin Hwang, Konstantinos Lymperopoulos
1Department of Physics and IRC in Bionanotechnology, Clarendon Laboratory, University of Oxford, Parks Road, Oxford, OX1 3PU, UK.
Methods in Molecular Biology (Clifton, N.J.)
|April 21, 2009
Abstract:
We present a single-molecule method for studying protein-DNA interactions based on fluorescence resonance energy transfer (FRET) and alternating-laser excitation (ALEX) of single diffusing molecules. An application of this method to the study of a bacterial transcription initiation complex is presented.

