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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Updated: Jun 23, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Microsomal proteomics.

Diana M Wong1, Khosrow Adeli

  • 1Molecular Structure and Function, the Hospital for Sick Children, Research Institute, University of Toronto, Toronto, ON, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|April 22, 2009
PubMed
Summary
This summary is machine-generated.

This study details methods for isolating microsomes, including the endoplasmic reticulum and Golgi apparatus, from liver and intestinal cells. Proteomic analysis of these compartments aids in understanding cellular functions.

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Area of Science:

  • Cell Biology
  • Proteomics
  • Organelle Biology

Background:

  • Proteomic profiling of subcellular compartments offers advantages over whole-cell lysate analysis.
  • Microsomes are key membranous organelles involved in protein synthesis, sorting, secretion, and metabolism.

Purpose of the Study:

  • To describe detailed methods for isolating microsomal organelles (ER, Golgi, PCTV) from primary hepatocytes and enterocytes.
  • To enable proteomic profiling of specific subcellular compartments for functional characterization.

Main Methods:

  • Isolation of microsomes from freshly isolated rodent hepatocytes and enterocytes.
  • Proteomic analysis using two-dimensional gel electrophoresis and mass spectrometry.

Main Results:

  • Successful isolation of microsomes, including ER, Golgi, and PCTV, from liver and intestinal cells.
  • Proteomic profiles of isolated microsomes were generated, facilitating organelle-specific functional insights.

Conclusions:

  • Subcellular fractionation and proteomic analysis provide a powerful approach to study organelle function.
  • This methodology is crucial for understanding lipoprotein assembly and secretion in intestinal cells.