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Two-dimensional Gel Electrophoresis01:22

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Creating 2DE databases for the World Wide Web.

Christine Hoogl1, Khaled Mostaguir, Ron D Appel

  • 1Swiss Institute of Bioinformatics, Proteome Informatics Group, CMU-1, 1 Rue Michel Servet, CH-1211, Geneva, 4, Switzerland.

Methods in Molecular Biology (Clifton, N.J.)
|April 22, 2009
PubMed
Summary
This summary is machine-generated.

This chapter details creating federated two-dimensional electrophoresis (2DE) databases for internet access. It explains preparing files for building and updating these integrated protein and gel image data resources.

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Area of Science:

  • Proteomics
  • Bioinformatics
  • Computational Biology

Background:

  • The internet has facilitated the creation of numerous two-dimensional electrophoresis (2DE) databases.
  • These databases integrate gel images and protein information, offering valuable data for research.

Purpose of the Study:

  • To provide a guide for preparing files to build a federated 2DE database.
  • To explain methods for making 2DE databases accessible via the internet.
  • To outline procedures for updating federated 2DE databases.

Main Methods:

  • Utilizing active hypertext links to connect gel images and protein data.
  • Preparing specific file formats for internet-based database construction.
  • Implementing strategies for ongoing database updates.

Main Results:

  • Demonstration of how to create a federated 2DE database structure.
  • Enabling data integration and navigation across linked databases.
  • Establishing a framework for accessible and up-to-date proteomic information online.

Conclusions:

  • Federated 2DE databases offer a powerful tool for integrating and accessing proteomic data.
  • The methods described facilitate the creation and maintenance of internet-accessible 2DE resources.
  • This approach enhances data sharing and utilization in the field of proteomics.