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Updated: Jun 23, 2026

Magnetic Resonance Spectroscopy of live Drosophila melanogaster using Magic Angle Spinning
Published on: April 15, 2010
Magnetic resonance spectroscopy of living tissues
Natalie J Serkova1, Kendra M Hasebroock, Susan L Kraft
1Department of Anesthesiology and Radiology, University of Colorado at Denver and Health Sciences Center, Aurora, CO, USA.
Abstract:
The comprehensive work of both clinical and basic science colleagues has demonstrated a clear proof of concept for "in vitro discovered- in vivo validated" biomarkers in translational metabolic profiling research using magnetic resonance techniques. Major tissue metabolites (initially discovered by high-resolution in vitro techniques on cancer specimens) can be translated into in vivo protocols based on noninvasive magnetic resonance spectroscopy (MRS). Using (1)H- and (31)P-MRS on living animals or patients, a decrease in citrate and polyamines in prostate cancer, an increase of cholines in breast cancer, as well as a decreased NAA and an increased lactate in gliomas during cancer progression can be assessed noninvasively. MRS can be used to follow up conventional cytotoxic as well as targeted anticancer therapies, which has been extensively done in animal models of cancer. This review focuses on applications and protocol development for in vivo (1)H- and (31)P-MRS on small animal models as well as on larger animals in cancer research, diagnosis, and treatment.
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