Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ventricular conduction is a marker for arrhythmic risk in SCN5A-E1784K overlap sodium channel disease.

Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology·2026
Same author

Clinical profile and prognosis of brugada syndrome SCN5A variant carriers with negative sodium channel blocker challenge.

Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology·2026
Same author

Identification of rare missense variants reducing cathepsin O secretion in families with intracranial aneurysm.

Cardiovascular research·2026
Same author

Domain mapping of disease mutations reveals pathogenic SORL1 variants in Alzheimer's disease.

Molecular neurodegeneration·2025
Same author

In vitro models to mimic tumor endothelial cell-mediated immune cell reprogramming in lung adenocarcinoma.

Journal of experimental & clinical cancer research : CR·2025
Same author

PERIGENOMED-CLINICS 1-the first study on feasibility, acceptability and psychosocial impact of PERIGENOMED: a pilot project aimed at providing initial concrete evidence on the relevance of panel-based genome sequencing for newborn screening (NBS) in France.

BMJ open·2025
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Jun 23, 2026

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization
16:37

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Published on: August 5, 2008

Comparative genomic hybridization: DNA preparation for microarray fabrication.

Richard Redon1, Diane Rigler, Nigel P Carter

  • 1Wellcome Trust, Sanger Institute, Cambridge, UK.

Methods in Molecular Biology (Clifton, N.J.)
|April 22, 2009
PubMed
Summary
This summary is machine-generated.

We developed scalable methods to create DNA microarrays using large-insert clones for improved genomic analysis. These techniques enhance the resolution of array-comparative genomic hybridization (array-CGH) for various applications.

More Related Videos

Array Comparative Genomic Hybridization (Array CGH) for Detection of Genomic Copy Number Variants
09:16

Array Comparative Genomic Hybridization (Array CGH) for Detection of Genomic Copy Number Variants

Published on: February 21, 2015

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants
09:32

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

Published on: November 8, 2017

Related Experiment Videos

Last Updated: Jun 23, 2026

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization
16:37

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Published on: August 5, 2008

Array Comparative Genomic Hybridization (Array CGH) for Detection of Genomic Copy Number Variants
09:16

Array Comparative Genomic Hybridization (Array CGH) for Detection of Genomic Copy Number Variants

Published on: February 21, 2015

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants
09:32

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

Published on: November 8, 2017

Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • The spatial resolution of microarray-based comparative genomic hybridization (array-CGH) is crucial for accurate genomic analysis.
  • Resolution is directly influenced by the length and density of DNA sequences on the microarray.
  • Existing methods may have limitations in achieving high resolution across all genomic regions.

Purpose of the Study:

  • To describe novel methods for constructing high-resolution DNA microarrays.
  • To utilize large-insert clones from genome sequencing projects for microarray development.
  • To create scalable protocols applicable to various clone types and array sizes.

Main Methods:

  • Development of protocols for constructing microarrays using large-insert clones (BAC, PAC, fosmid, cosmid).
  • Application of methods at the Wellcome Trust Sanger Institute.
  • Ensuring scalability for microarrays ranging from hundreds to tens of thousands of clones.

Main Results:

  • Successfully developed and validated methods for microarray construction using large-insert DNA clones.
  • Demonstrated applicability to Bacterial Artificial Chromosomes (BAC), Phage Artificial Chromosomes (PAC), fosmids, and cosmids.
  • Established scalable protocols capable of producing microarrays with diverse clone numbers.

Conclusions:

  • The described methods enable the creation of high-resolution DNA microarrays.
  • These microarrays are valuable tools for advancing array-CGH and other genomic studies.
  • The scalable protocols support the construction of custom microarrays for specific research needs.