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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Updated: Jun 23, 2026

Performing Custom MicroRNA Microarray Experiments
07:04

Performing Custom MicroRNA Microarray Experiments

Published on: October 28, 2011

Introduction to microarray technology.

Martin Dufva1

  • 1Technical University of Denmark, Kgs, Lyngby, Denmark.

Methods in Molecular Biology (Clifton, N.J.)
|April 22, 2009
PubMed
Summary
This summary is machine-generated.

DNA microarrays enable high-throughput analysis of millions of molecules simultaneously. This technology integrates diverse fields for applications like genotyping and gene expression profiling.

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DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning
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Last Updated: Jun 23, 2026

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Bioinformatics

Background:

  • DNA microarray technology offers high-throughput analysis for numerous applications.
  • It enables simultaneous probing of millions of molecules, driving its rapid adoption.
  • The technique integrates diverse fields including microfabrication, chemistry, optics, and bioinformatics.

Purpose of the Study:

  • To introduce the fundamental concepts and nomenclature of microarray technology.
  • To provide an overview of the five basic steps involved in microarray analysis.
  • To explain the interdisciplinary nature of microarray development and application.

Main Methods:

  • Fabrication of microarray platforms.
  • Preparation of target nucleic acid samples.
  • Hybridization of targets to probes on the array.
  • Detection of hybridization signals.
  • Bioinformatic analysis of generated data.

Main Results:

  • Microarray technology facilitates large-scale genotyping, gene expression profiling, and comparative genomic hybridization.
  • The process involves intricate steps from fabrication to data analysis.
  • Understanding the basic principles is crucial for effective application.

Conclusions:

  • Microarray technology is a powerful, interdisciplinary tool for molecular analysis.
  • Its core steps include fabrication, target prep, hybridization, detection, and data analysis.
  • This chapter serves as a foundational guide to microarray principles and practices.