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Related Experiment Video

Updated: Jun 23, 2026

Three-dimensional Alginate-bead Culture of Human Pituitary Adenoma Cells

Three-dimensional Alginate-bead Culture of Human Pituitary Adenoma Cells

Published on: February 18, 2016

Rab3B immunoexpression in human pituitary adenomas.

Fabio Rotondo¹, Bernd W Scheithauer, Kalman Kovacs

¹Department of Laboratory Medicine, Division of Pathology, St Michael's Hospital, University of Toronto, Toronto, Ontario, Canada. rotondof@smh.toronto.on.ca

Applied Immunohistochemistry & Molecular Morphology : AIMM

|April 23, 2009

Summary

Rab3B protein is present in human pituitary adenomas, with varying levels across tumor types. Its expression is highest in corticotroph adenomas and is affected by bromocriptine treatment.

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Last Updated: Jun 23, 2026

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Published on: February 18, 2016

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Area of Science:

Endocrinology
Cell Biology
Oncology

Background:

Rab3B is a GTP-binding protein involved in exocytosis regulation.
Its role in normal human pituitary and pituitary adenomas requires further elucidation.

Purpose of the Study:

To investigate the presence and distribution of Rab3B in various human pituitary adenomas.
To assess the impact of specific therapeutic agents on Rab3B expression in pituitary tumors.

Main Methods:

Immunohistochemical analysis of 130 surgically removed human pituitary adenomas.
Inclusion of 10 non-tumorous adenohypophyses for comparison.
Tumor classification based on WHO criteria (histologic, immunohistochemical, ultrastructural).

Main Results:

Rab3B immunopositivity was strongest in corticotroph adenomas, followed by thyrotroph, lactotroph, gonadotroph, null cell, and somatotroph adenomas.
Bromocriptine treatment correlated with decreased Rab3B immunoexpression; octreotide had no significant effect.
Cytoplasmic and evenly distributed Rab3B staining was observed, with no staining in normal adenohypophyses.

Conclusions:

Rab3B is differentially expressed across pituitary adenoma subtypes.
Rab3B is implicated in the regulation of pituitary hormone secretion.
Therapeutic interventions can modulate Rab3B expression in pituitary tumors.