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Updated: Jun 17, 2026

Harmonic Nanoparticles for Regenerative Research

Harmonic Nanoparticles for Regenerative Research

Published on: May 1, 2014

Third harmonic generation microscopy.

J Squier, M Muller, G Brakenhoff

|April 23, 2009

Summary

This summary is machine-generated.

Third harmonic generation (THG) microscopy now images living systems dynamically. This new method provides clear, non-fading images of plant activity, showing cells remain viable during observation.

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Area of Science:

Biophysics
Microscopy
Plant Biology

Background:

Traditional microscopy techniques often struggle to capture dynamic biological processes in living organisms without causing damage or photobleaching.
The development of advanced imaging modalities is crucial for understanding cellular dynamics and long-term biological activity.

Purpose of the Study:

To demonstrate the capability of third harmonic generation (THG) microscopy for real-time, dynamical imaging of living biological systems.
To assess the viability of specimens under THG imaging conditions.

Main Methods:

Utilized a 100 fs excitation pulse at 1.2 µm wavelength to generate a 400 nm signal directly within the specimen.
Applied THG microscopy to image Chara plant rhizoids.

Main Results:

Successfully generated dynamical images of living Chara plant rhizoids, revealing active biological processes.
Observed non-fading image characteristics during continuous viewing, indicating minimal phototoxicity.
Demonstrated prolonged specimen viability under THG-imaging conditions.

Conclusions:

Third harmonic generation microscopy is a viable technique for dynamical imaging of living systems.
THG microscopy offers a non-invasive imaging approach that preserves specimen viability for extended observation periods.
This technique opens new avenues for studying dynamic biological processes in real-time.