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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Jun 23, 2026

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Protein microarrays: high-throughput tools for proteomics.

Oda Stoevesandt¹, Michael J Taussig, Mingyue He

¹Babraham Bioscience Technologies Ltd., Babraham Research Campus, Cambridge, CB22 3AT, UK. oda.stoevesandt@bbsrc.ac.uk

Expert Review of Proteomics

|April 24, 2009

Summary

This summary is machine-generated.

Protein microarrays enable efficient, high-throughput screening of protein binding events. Recent advances allow their creation via in situ cell-free synthesis from DNA arrays, expanding proteomics and diagnostics applications.

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Last Updated: Jun 23, 2026

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

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Area of Science:

Biochemistry
Molecular Biology
Biotechnology

Background:

Protein microarrays are powerful tools for analyzing large numbers of proteins simultaneously.
They facilitate high-throughput screening of protein interactions, expression profiling, and target discovery.
Conventional methods involve spotting purified proteins, which can be time-consuming and costly.

Purpose of the Study:

To review recent technological advancements in protein microarray generation.
To highlight the diverse applications of protein microarrays in proteomics and diagnostics.
To discuss the shift towards in situ synthesis methods for protein microarray production.

Main Methods:

Review of recent literature on protein microarray technologies.
Analysis of methods for in situ cell-free synthesis of proteins on arrays.
Examination of case studies demonstrating microarray applications.

Main Results:

In situ cell-free synthesis offers a novel and efficient approach to protein microarray generation.
Protein microarrays are increasingly vital for complex proteomic analyses.
The technology shows significant promise for diagnostic applications.

Conclusions:

Advancements in protein microarray generation, particularly in situ synthesis, are transforming proteomics.
Protein microarrays are essential for high-throughput analyses and hold great potential in diagnostics.
Continued innovation in this field will further enhance their utility in biological research.