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Vimentin regulates scribble activity by protecting it from proteasomal degradation
Dominic C Y Phua1, Patrick O Humbert, Walter Hunziker
1Epithelial Cell Biology Laboratory, Cancer and Developmental Cell Biology Division, Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, Singapore 138673, Republic of Singapore.
Molecular Biology of the Cell
|April 24, 2009
Summary
Scribble (Scrib) protein interacts with vimentin, a key intermediate filament. This interaction is crucial for cell migration and wound healing, with vimentin stabilizing Scrib.
Area of Science:
- Cell Biology
- Cytoskeletal Dynamics
- Epithelial Cell Biology
Background:
- Scribble (Scrib), Discs large, and Lethal giant larvae form a complex regulating cell polarity.
- Cell polarity is essential for epithelial and migrating cell functions.
Purpose of the Study:
- To investigate the interaction between Scribble (Scrib) and the intermediate filament cytoskeleton.
- To elucidate the role of Scrib and vimentin in cell migration, polarization, and aggregation.
Main Methods:
- Utilized Madin-Darby canine kidney (MDCK) and human umbilical vein endothelial cells.
- Employed RNA interference to silence Scrib and/or vimentin.
- Observed Golgi polarization, cell migration, wound healing, and cell aggregation.
Main Results:
- Scrib binds to vimentin, redistributing to the plasma membrane during cell-cell contact formation.
- Silencing Scrib or vimentin impairs Golgi polarization during migration and delays wound healing.
- Cell aggregation is dependent on both Scrib and vimentin, with vimentin silencing increasing Scrib degradation.
Conclusions:
- Scrib and vimentin function coordinately in cell migration and aggregation.
- Vimentin stabilizes Scrib, suggesting its upregulation during epithelial-mesenchymal transitions promotes directed cell migration.
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