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Updated: Jun 23, 2026
12:54
Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
Published on: October 2, 2021
Development of an imaging modality utilizing 2D optical signals during an EPI-fluorescent optical mapping experiment
Phillip Prior1, Bradley J Roth
1Department of Physics, Oakland University, Rochester, MI 48309, USA. phil.prior@gmail.com
Physics in Medicine and Biology
|April 24, 2009
Summary
This study introduces a novel optical mapping technique using multiple wavelengths to image cardiac electrical activity. The method reconstructs transmembrane potential, offering new insights into defibrillation shock effects.
Area of Science:
- Biomedical Engineering
- Biophysics
- Cardiology
Background:
- Optical mapping visualizes heart electrical activity.
- Imaging transmembrane potential is crucial for studying defibrillation effects.
Purpose of the Study:
- To develop an alternative optical mapping method for imaging cardiac electrical activity.
- To utilize multiple wavelengths and optical decay constants for enhanced imaging.
Main Methods:
- Derived a modified diffusion equation Green's function for semi-infinite tissue.
- Related detected optical signals to photon emission sources.
- Reconstructed images using Gaussian quadrature and matrix inversion.
Main Results:
- Successfully imaged sources below the tissue surface.
- Demonstrated noise reduction using singular value decomposition.
- Identified the need for regularization methods for deep or localized sources.
Conclusions:
- The presented work demonstrates the feasibility of a new optical mapping imaging technique for cardiac electrical activity.
- This method offers potential for improved visualization of electrical phenomena in the heart.
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