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Updated: Jun 23, 2026

Quantification of Reactive Oxygen Species Using 2′,7′-Dichlorofluorescein Diacetate Probe and Flow-Cytometry in Müller Glial Cells
Published on: May 13, 2022
Reactive oxygen species in diabetes-induced vascular damage, stroke, and Alzheimer's disease
Sajjad Muhammad1, Angelika Bierhaus, Markus Schwaninger
1Institute of Pharmacology, Department of Internal Medicine, University of Heidelberg, Heidelberg, Germany.
Abstract:
A morphological hallmark of Alzheimer's disease (AD) is the deposition of amyloid-beta peptide in plaques and along blood vessels. As several lines of evidence suggest that vascular dysfunction contributes to AD, the pathophysiology of diabetic vasculopathy and stroke may cast light on the vascular component of AD. In this review, we compile some recent findings on the role of reactive oxygen species in diabetes-induced vascular dysfunction and the consequent cerebral ischemia and compare them with key findings in AD. Overall, there is compelling evidence that reactive oxygen species play a key role in the pathophysiology of AD. Unfortunately, this insight has not yet led to a new treatment of AD.
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