Real time intraoperative confocal laser microscopy-guided surgery
Nam Q Nguyen1, Andrew V Biankin, Rupert W L Leong
1Cancer Research Program, Garvan Institute of Medical Research, Sydney, New South Wales, Australia.
Objective:
To assess the potential utility of in vivo histologic surface and subsurface imaging in real-time using the Optiscan confocal laser microscope to detect diseased tissue at the time of surgery.
Summary Background Data:
The goal of surgical treatment of diseases such as cancer is complete microscopic resection of diseased tissue; however, current methods for the assessment of extent of disease at the time of surgery are inadequate.
Methods:
We assessed the potential of the Optiscan confocal laser microscope, a new device developed for real-time in vivo histologic surface and subsurface imaging during surgery.
Results:
Intravenous Fluorescein Sodium contrast enabled visualization of cellular and architectural morphology of intra-abdominal organs with magnification equivalent to light microscopy and enabled differentiation between normal organs and disease.
Conclusions:
Real time intraoperative confocal microscopy has significant potential application in detecting disease, and influencing decision-making at the time of surgery.
Insights
Real-time confocal microscopy using the Optiscan device can visualize tissue at the cellular level during surgery. This technology shows potential for detecting diseased tissue and guiding surgical decisions.
Area of Science:
- Surgical Oncology
- Medical Imaging Technology
- Histopathology
Background:
- Complete microscopic resection of diseased tissue is the goal of surgical treatment for diseases like cancer.
- Current intraoperative methods for assessing disease extent are insufficient.
- Accurate real-time assessment of tissue during surgery is crucial for effective treatment.
Purpose of the Study:
- To evaluate the utility of the Optiscan confocal laser microscope for real-time in vivo histologic imaging.
- To determine if this technology can detect diseased tissue during surgical procedures.
- To assess the potential of intraoperative confocal microscopy in guiding surgical decisions.
Main Methods:
- The Optiscan confocal laser microscope was utilized for real-time in vivo histologic surface and subsurface imaging.
- Intravenous Fluorescein Sodium was administered as a contrast agent.
- Imaging was performed during surgical procedures to assess intra-abdominal organs.
Main Results:
- The Optiscan microscope provided visualization of cellular and architectural morphology comparable to light microscopy.
- Fluorescein Sodium contrast enhanced the differentiation between normal and diseased tissues.
- The system enabled real-time imaging of tissue during surgery.
Conclusions:
- Real-time intraoperative confocal microscopy demonstrates significant potential for disease detection during surgery.
- This technology can aid in making critical decisions at the time of surgery.
- The Optiscan confocal laser microscope offers a promising tool for improving surgical outcomes.
