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Competitive binding of cadmium by plant thiols: an electrochemical study assisted by multivariate curve resolution
Rui Gusmão1, Cristina Ariño, José Manuel Díaz-Cruz
1Departament de Química Analítica, Universitat de Barcelona, Martí i Franquès 1-11, 08028, Barcelona, Spain.
Abstract:
Multivariate curve resolution with alternating least squares (MCR-ALS) has been applied to voltammetric data obtained from analysis of the competitive binding of cysteine (Cys) and cysteine-glycine (Cys-Gly) by Cd(II) as a first approach towards mixtures of phytochelatins and related compounds in natural media. From different starting points, the possibilities of formation of mixed complexes and/or displacements between ligands are investigated. Analysis of the resulting unitary voltammograms and concentration profiles of the resolved components by MCR-ALS suggests that the strongest ligand (Cys-Gly) is able to displace the weakest (Cys) from its metal complexes, whereas this does not happen in the opposite direction. On the other hand, no evidence of Cd mixed-ligand complexes was found.
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