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Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells
Published on: August 9, 2019
Multipotent progenitor cells are present in human peripheral blood.
Daniela Cesselli1, Antonio Paolo Beltrami, Silvia Rigo
1Center for Regenerative Medicine, University of Udine, Italy.
Circulation Research
|April 25, 2009
Summary
Multipotent progenitor cells (MPCs) were isolated from human peripheral blood after granulocyte colony-stimulating factor administration. These cells exhibit embryonic stem cell-like properties and can differentiate into various cell types, offering potential for clinical applications.
Area of Science:
- Stem Cell Biology
- Regenerative Medicine
- Hematology
Background:
- Human peripheral blood may harbor multipotent progenitor cells (MPCs).
- Granulocyte colony-stimulating factor (G-CSF) administration mobilizes cells from bone marrow to peripheral blood.
Purpose of the Study:
- To determine if multipotent progenitor cells (MPCs) exist in human peripheral blood.
- To characterize the properties and differentiation potential of these peripheral blood MPCs.
Main Methods:
- Leukapheresis products from G-CSF-treated donors were analyzed.
- Adherent proliferating cells were cultured and expanded.
- Phenotypic characterization using stem cell and lineage markers was performed.
- In vitro differentiation assays and in vivo transplantation studies were conducted.
Main Results:
- Expanded cells exhibited long-term proliferation without senescence and expressed key pluripotency transcription factors (Nanog, Oct3/4, Sox2, c-Myc, Klf4).
- Cells expressed mesenchymal stem cell antigens but lacked hematopoietic markers.
- MPCs demonstrated multipotency, differentiating into neurons, glial cells, hepatocytes, cardiomyocytes, endothelial cells, and osteoblasts.
- Transplantation in mice showed MPCs migrated, integrated, and differentiated into resident parenchymal cells.
Conclusions:
- Undifferentiated cells with embryonic stem cell-like properties can be isolated and expanded from human peripheral blood post-G-CSF treatment.
- These peripheral blood MPCs represent a promising source of autologous cells for therapeutic purposes.
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