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Related Concept Videos

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Transcription Attenuation in Prokaryotes

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Related Experiment Video

Updated: Jun 23, 2026

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
11:34

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

SVOP is a nucleotide binding protein.

Jia Yao1, Sandra M Bajjalieh

  • 1Department of Pharmacology, University of Washington, Seattle, Washington, United States of America.

Plos One
|April 25, 2009
PubMed
Summary
This summary is machine-generated.

Synaptic vesicle related protein (SVOP) binds nucleotides, similar to SV2 and Glut1. However, SVOP has a unique binding site and higher affinity for NAD, suggesting independent evolution of nucleotide binding in this protein family.

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Last Updated: Jun 23, 2026

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Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach
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In Vitro Transcription Assays and Their Application in Drug Discovery
09:28

In Vitro Transcription Assays and Their Application in Drug Discovery

Published on: September 20, 2016

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Protein Biochemistry

Background:

  • Synaptic vesicle protein 2 (SV2) and SVOP are transporter-like proteins in neurotransmitter vesicles.
  • Both SV2 and SVOP share structural similarities with major facilitator (MF) family transporters.
  • SV2 is known to bind nucleotides, influencing its transport activity.

Purpose of the Study:

  • To determine if SVOP binds nucleotides.
  • To characterize the nucleotide binding properties of SVOP.
  • To compare SVOP's nucleotide binding to that of SV2 and Glut1.

Main Methods:

  • In vitro photoaffinity labeling using 8-azido-ATP[gamma] biotin.
  • Purification of recombinant SVOP-FLAG fusion protein.
  • Dose-dependent nucleotide inhibition studies and binding site mapping.

Main Results:

  • SVOP was confirmed as a nucleotide-binding protein.
  • SVOP exhibits distinct substrate specificity and binding site compared to SV2.
  • SVOP shows the highest affinity for NAD, unlike SV2 which binds NAD and ATP equally.
  • The SVOP nucleotide binding site is located in transmembrane domains 9-12.

Conclusions:

  • SVOP is the third MF family member identified as a nucleotide-binding protein.
  • The unique nucleotide binding sites in SVOP, SV2, and Glut1 suggest independent evolutionary origins.
  • This finding expands our understanding of nucleotide interactions within the MF transporter family.