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Related Concept Videos

In vitro Mutagenesis01:16

In vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
Gene Conversion02:08

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

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Related Experiment Video

Updated: Jun 23, 2026

Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis
08:36

Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Published on: July 16, 2019

Regional mutagenesis using Dissociation in maize.

Kevin R Ahern1, Prasit Deewatthanawong, Justin Schares

  • 1Boyce Thompson Institute for Plant Research, Ithaca, NY 14853, USA.

Methods (San Diego, Calif.)
|April 28, 2009
PubMed
Summary
This summary is machine-generated.

New genetic tools and methods now allow Dissociation (Ds) to be used as an insertional mutagen in maize. These resources accelerate the study of gene function through the creation and identification of Ds insertion alleles.

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Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes
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Published on: February 14, 2020

Related Experiment Videos

Last Updated: Jun 23, 2026

Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis
08:36

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Published on: July 16, 2019

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes
10:28

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

Published on: February 14, 2020

Area of Science:

  • Plant genetics
  • Molecular biology
  • Genomics

Background:

  • The Dissociation (Ds) element is a valuable tool for genetic manipulation in maize.
  • Efficient methods for generating and identifying Ds insertions are crucial for functional genomics.

Purpose of the Study:

  • To introduce novel genetic screens, molecular methods, and web resources for utilizing Ds as an insertional mutagen in maize.
  • To facilitate the identification and characterization of Ds insertions in genes-of-interest (goi).

Main Methods:

  • Development of two genetic screens (Scheme I and Scheme II) for identifying Ds insertions.
  • Implementation of an inverse PCR protocol for amplifying sequences flanking Ds insertions.
  • Establishment of a high-throughput 96-well plate DNA extraction method.
  • Creation of web-based tools for accessing genetic materials.

Main Results:

  • Over 1700 Ds elements distributed across the maize genome for mutagenesis.
  • Successful demonstration of Ds insertion generation for gene cloning and allelic series creation.
  • Efficient PCR-based screening for rare Ds insertion alleles in testcross progeny.
  • Availability of robust molecular protocols and web resources.

Conclusions:

  • The newly developed Ds insertion lines and associated methods significantly accelerate functional genomics research in maize.
  • These resources provide powerful tools for gene discovery and characterization in maize.
  • The integrated approach enhances the utility of Ds elements for genetic studies.