Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 23, 2026

Tracing de novo Lipids using Stable Isotope Labeling LC-TIMS-TOF MS/MS
07:12

Tracing de novo Lipids using Stable Isotope Labeling LC-TIMS-TOF MS/MS

Published on: August 23, 2024

Dynamic lipidomics with stable isotope labelling.

Anthony D Postle1, Alan N Hunt

  • 1Division of Infection, Inflammation & Immunity, University of Southampton, School of Medicine, Southampton General Hospital, Mailpoint 803, Level F, South Block, Tremona Road, Southampton SO16 6YD, UK. adp@soton.ac.uk

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
|April 28, 2009
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effects of Sample Deposition Medium and Drying on Spectroscopic Quantification of Lipid Biomarkers in Respiratory Distress Syndrome.

Biosensors·2026
Same author

Endotracheal surfactant for infants with life-threatening bronchiolitis (BESS): a randomised, blinded, sham-controlled, phase 2 trial.

The Lancet. Respiratory medicine·2026
Same author

Surfactant nebulization in severe COVID-19: Tracheal aspirate phospholipid turnover and metabolism.

Biochimica et biophysica acta. Molecular and cell biology of lipids·2026
Same author

Hyperoxia and surfactant dysfunction in critical illness: insights and future therapeutic prospects.

American journal of respiratory cell and molecular biology·2025
Same author

Surfactant Phospholipid Kinetics in Ventilated Children after Therapeutic Surfactant Supplementation.

International journal of molecular sciences·2024
Same author

Oxidative stress, redox status and surfactant metabolism in mechanically ventilated patients receiving different approaches to oxygen therapy (MecROX): An observational study protocol for mechanistic evaluation.

NIHR open research·2024
Same journal

EGCG alleviates sepsis-induced myocardial injury by regulating macrophage polarization via the TLR4/MyD88/NF-κB pathway.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2026
Same journal

Hydrogen-bonded organic frameworks-immobilized thrombin: Ligand fishing of thrombin inhibitors from Chishao and Chishao-Chuanxiong herbal pair.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2026
Same journal

Integrated network pharmacology, gut microbiome, and metabolomics analyses reveal the protective mechanism of Guizhi Gegen decoction against influenza.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2026
Same journal

Identification of 11α/β-hydroxy-12β-deoxysaxitoxin (12β-deoxyM2) in bivalve molluscs.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2026
Same journal

High-resolution LC-MS/MS analysis of brain N-glycans reveals composition-specific changes in Parkinson's disease.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2026
Same journal

The discovery of α-glucosidase inhibitors from Folium Mori using virtual screening and ligand fishing strategies.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2026
See all related articles

Stable isotope labeling allows dynamic lipidomics to track lipid synthesis and turnover. Tandem mass spectrometry can now measure intact lipid substrates, offering new insights into metabolic diseases.

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Metabolomics

Background:

  • Stable isotope labeling is crucial for studying lipid metabolism.
  • Dynamic lipidomics provides insights into lipid synthesis and turnover.
  • Tandem mass spectrometry has advanced lipid analysis.

Purpose of the Study:

  • To detail a method for dynamic lipidomics using stable isotope labeling.
  • To demonstrate the analysis of intact lipid substrates.
  • To explore the potential for probing lipid metabolism diseases.

Main Methods:

  • Utilizing stable isotope labeled precursors (deuterated choline, ethanolamine, inositol).
  • Employing tandem mass spectrometry with precursor and neutral loss scans.
  • Analyzing phosphatidylcholine, phosphatidylethanolamine, and phosphatidylinositol.

More Related Videos

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
09:26

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis

Published on: November 17, 2011

Shotgun Lipidomics of Rodent Tissues
11:46

Shotgun Lipidomics of Rodent Tissues

Published on: November 18, 2022

Related Experiment Videos

Last Updated: Jun 23, 2026

Tracing de novo Lipids using Stable Isotope Labeling LC-TIMS-TOF MS/MS
07:12

Tracing de novo Lipids using Stable Isotope Labeling LC-TIMS-TOF MS/MS

Published on: August 23, 2024

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
09:26

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis

Published on: November 17, 2011

Shotgun Lipidomics of Rodent Tissues
11:46

Shotgun Lipidomics of Rodent Tissues

Published on: November 18, 2022

Main Results:

  • Quantification of isotope enrichments in lipids.
  • Determination of intact lipid substrates.
  • Measurement of kinetics for individual phospholipid molecular species.

Conclusions:

  • This approach enables dynamic lipidomics in clinical and experimental settings.
  • It provides detailed kinetic information on specific phospholipid species.
  • Has significant potential for investigating in vivo lipid metabolism disorders.