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Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Related Experiment Video

Updated: Jun 23, 2026

Generation of Transgenic Rats using a Lentiviral Vector Approach
09:07

Generation of Transgenic Rats using a Lentiviral Vector Approach

Published on: May 17, 2020

Generating mutant rats using the Sleeping Beauty transposon system.

Kazuhiro Kitada1, Vincent W Keng, Junji Takeda

  • 1Laboratory of Mammalian Genetics, Division of Genome Dynamics, Creative Research Initiative "Sousei", Hokkaido University, North 10 West 8, Kita-ku, Sapporo 060-0810, Japan.

Methods (San Diego, Calif.)
|April 29, 2009
PubMed
Summary

Researchers developed a fast method to create mutant rats for biomedical research using the Sleeping Beauty (SB) transposon system. This technique enables large-scale generation of genetically modified rats, expanding valuable research resources.

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Last Updated: Jun 23, 2026

Generation of Transgenic Rats using a Lentiviral Vector Approach
09:07

Generation of Transgenic Rats using a Lentiviral Vector Approach

Published on: May 17, 2020

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Identification of Sleeping Beauty Transposon Insertions in Solid Tumors using Linker-mediated PCR

Published on: February 1, 2013

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08:44

Generation of Topically Transgenic Rats by In utero Electroporation and In vivo Bioluminescence Screening

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Area of Science:

  • Genetics
  • Molecular Biology
  • Animal Models

Background:

  • Laboratory rats are crucial for biomedical research.
  • Limited availability of mutant rat resources hinders research progress.
  • Developing efficient methods for generating mutants is essential.

Purpose of the Study:

  • To establish a rapid method for generating insertional mutant rats.
  • To leverage the Sleeping Beauty (SB) transposon system for large-scale mutant generation.
  • To create a valuable resource for the biomedical research community.

Main Methods:

  • Generated transgenic rats carrying SB transposon vector and SB transposase.
  • Interbred rats to obtain doubly-transgenic individuals.
  • Mobilized the SB transposon in the germline for genomic reinsertion.
  • Utilized green fluorescence protein (GFP) reporter for non-invasive identification of gene insertion events.
  • Confirmed gene mutations using linker ligation-mediated PCR or 3'-rapid amplification of cDNA ends (3'RACE).
  • Employed a LacZ gene for visualizing endogenous expression profiles of mutated genes.

Main Results:

  • Successfully generated heterozygous mutant rats.
  • Demonstrated a rapid and non-invasive method for identifying gene insertions via GFP reporter.
  • Confirmed gene mutations using established molecular techniques.
  • Showcased the utility of the LacZ reporter for expression analysis.

Conclusions:

  • The SB transposon system provides a straightforward and efficient method for generating insertional mutant rats.
  • This approach is readily adaptable to other transposon systems.
  • The developed method will significantly contribute to the availability of mutant rat resources for biomedical research.