Related Experiment Video
Updated: Jun 23, 2026
A Galleria mellonella Oral Administration Model to Study Commensal-Induced Innate Immune Responses
Published on: March 21, 2019
Effect of exogenous nitric oxide on murine splenic immune response induced by Aggregatibacter actinomycetemcomitans
W Sosroseno1, P S Bird, G J Seymour
1School of Dentistry, AIMST University, Semeling, Bedong 08100, Kedah Darul Aman, Malaysia. wsosroseno@yahoo.com
Abstract:
The aim of this study was to determine the effect of exogenous nitric oxide (NO) on the induction of murine splenic immune response to Aggregatibacter actinomycetemcomitans lipopolysaccharide (LPS) in vitro. BALB/c mice were immunized with A. actinomycetemcomitans LPS and a control group was sham-immunized. Spleen cells were obtained, cultured and stimulated with A. actinomycetemcomitans LPS with or without the presence of S-nitroso acetyl-penicillamine (SNAP), a NO donor, and carboxy-PTIO, an NO scavenger. Culture supernatants were assessed for inducible nitric oxide synthase (iNOS) activity, specific IgG subclass levels, and both IFN-gamma and IL-4 levels. The results showed that in A. actinomycetemcomitans LPS-stimulated cells, SNAP enhances iNOS activity but inhibits the levels of specific IgG2a and IFN-gamma suggesting a Th1 response. The effect of SNAP on these immune parameters was ablated by carboxy-PTIO. These results suggest that exogenous NO may suppress the Th1-like immune response of A. actinomycetemcomitans LPS-stimulated murine spleen cells.
