Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For example, the mass of helium...
¹H NMR of Conformationally Flexible Molecules: Temporal Resolution00:52

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are slanted or...
Racemic Mixtures and the Resolution of Enantiomers02:30

Racemic Mixtures and the Resolution of Enantiomers

A racemic mixture, or racemate, is an equimolar mixture of enantiomers of a molecule that can be separated using their unique interaction with chiral molecules or media. Racemic mixtures are denoted by the (±)- prefix. This ‘optical rotation descriptor’ applies to the whole solution of a racemic mixture rather than a specific stereoisomer. Enantiomers typically have the same physical and chemical properties. Hence, they are not easily separable. However, enantiomers can exhibit different...
Stereoisomerism02:52

Stereoisomerism

Isomerism in Complexes
Isomers are different chemical species that have the same chemical formula.
Transition metal complexes often exist as geometric isomers, in which the same atoms are connected through the same types of bonds but with differences in their orientation in space. Coordination complexes with two different ligands in the cis and trans positions from a ligand of interest form isomers. For example, the octahedral [Co(NH3)4Cl2]+ ion has two isomers (Figure 1) In the cis...
Mass Analyzers: Overview01:13

Mass Analyzers: Overview

The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Robust single-scan ultraselective NMR.

Chemical communications (Cambridge, England)·2026
Same author

Binary colloidal mixtures in near-critical binary solvents.

The Journal of chemical physics·2026
Same author

General Nuclear Magnetic Resonance Analysis Toolbox for Stats: A Comprehensive Module for Nuclear Magnetic Resonance-Based Chemometrics and Metabolomics.

Analytical chemistry·2026
Same author

Practical Guide and Best Practices for Diffusion NMR Processing With GNAT.

Magnetic resonance in chemistry : MRC·2026
Same author

Urinary tract infections after benign cystectomy: Incidence, risk factors, pathogens, and resistance patterns.

Investigative and clinical urology·2026
Same author

Real-Time NMR Quantification of Paramagnetic Species during Chemical Reactions.

Analytical chemistry·2026
Same journal

Biodegradable Self-Powered Electrotherapy Patch for Integrated Smart Wound Management.

Analytical chemistry·2026
Same journal

Metabolite Fraction Libraries for Quantitative NMR Metabolomics.

Analytical chemistry·2026
Same journal

Self-Contained Lateral-Flow Microfluidic Bead-Based Assay for Rapid Quantification of Early-Stage Kidney Biomarkers.

Analytical chemistry·2026
Same journal

Overcoming the Debye Shielding Effect with Concave-Convex Structures for Sensitivity-Enhanced Thin-Film Transistors.

Analytical chemistry·2026
Same journal

Mode-Phase-Difference Photothermal Spectroscopy Assisted by a Bent Biconically Tapered Microfiber for Gas Sensing.

Analytical chemistry·2026
Same journal

Negative-Pressure-Actuated Microfluidics: A Dual-Mode Point-of-Care Sensor for Allergen-Specific IgE in Interstitial Fluid.

Analytical chemistry·2026
See all related articles

Related Experiment Video

Updated: Jun 23, 2026

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
08:40

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

Published on: January 20, 2022

Isomer resolution by micelle-assisted diffusion-ordered spectroscopy.

Robert Evans1, Stephan Haiber, Mathias Nilsson

  • 1School of Chemistry, University of Manchester, Oxford Road, Manchester M13 9PL, United Kingdom.

Analytical Chemistry
|May 1, 2009
PubMed
Summary
This summary is machine-generated.

Diffusion-ordered NMR spectroscopy (DOSY) can now distinguish isomers using surfactant micelles. This micelle-assisted DOSY technique enhances mixture component identification for challenging samples.

More Related Videos

Determination of the Photoisomerization Quantum Yield of a Hydrazone Photoswitch
09:33

Determination of the Photoisomerization Quantum Yield of a Hydrazone Photoswitch

Published on: February 7, 2022

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species
08:53

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

Published on: May 7, 2018

Related Experiment Videos

Last Updated: Jun 23, 2026

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
08:40

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

Published on: January 20, 2022

Determination of the Photoisomerization Quantum Yield of a Hydrazone Photoswitch
09:33

Determination of the Photoisomerization Quantum Yield of a Hydrazone Photoswitch

Published on: February 7, 2022

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species
08:53

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

Published on: May 7, 2018

Area of Science:

  • Analytical Chemistry
  • Physical Chemistry
  • Spectroscopy

Background:

  • Diffusion-ordered NMR spectroscopy (DOSY) is a valuable technique for identifying components in mixtures.
  • Basic DOSY relies on differences in hydrodynamic radius, which is often insufficient for distinguishing isomers.
  • Isomeric compounds possess identical molecular formulas but differ in atomic arrangement, posing challenges for traditional separation and identification methods.

Purpose of the Study:

  • To demonstrate the utility of micelle-assisted DOSY for resolving NMR spectra of isomeric compounds.
  • To showcase the application of this technique in both aqueous and non-aqueous solutions.
  • To expand the capabilities of DOSY for complex mixture analysis.

Main Methods:

  • Utilized micelle-assisted Diffusion-ordered NMR spectroscopy (DOSY).
  • Employed sodium dodecyl sulfate (SDS) micelles in aqueous solutions.
  • Used AOT (bis(2-ethylhexyl) sulfosuccinate sodium salt) reversed micelles in chloroform solutions.
  • Investigated the separation of three dihydroxybenzene isomers: catechol, resorcinol, and hydroquinone.

Main Results:

  • Micelle-assisted DOSY successfully distinguished between isomeric dihydroxybenzenes.
  • Different degrees of interaction with micelles and reversed micelles allowed for spectral resolution.
  • The technique proved effective in both aqueous (SDS) and non-aqueous (AOT) environments.
  • Enhanced spectral clarity was achieved for complex isomeric mixtures.

Conclusions:

  • Micelle-assisted DOSY is a powerful extension of traditional DOSY for isomer identification.
  • This method overcomes the limitations of basic DOSY in differentiating molecules with similar hydrodynamic radii.
  • The approach offers a versatile strategy for analyzing complex mixtures containing isomers in various solvent systems.