Related Experiment Video
Updated: Jun 23, 2026

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
Published on: December 15, 2017
Optimization of medium composition for butyric acid production by Clostridium thermobutyricum using response surface
Chun-Hui Zhang1, Yu-Jiu Ma, Fang-Xiao Yang
1Key Laboratory of Biofuel, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Songling road 189, Qingdao 266071, China.
Abstract:
The optimal medium for butyric acid production by Clostridium thermobutyricum in a shake flask culture was studied using statistical experimental design and analysis. The optimal composition of the fermentation medium for maximum butyric acid yield, as determined on the basis of a three-level four-factor Box-Behnken design (BBD), was obtained by response surface methodology (RSM). The high correlation between the predicted and observed values indicated the validity of the model. A maximum butyric acid yield of 12.05 g/l was obtained at K(2)HPO(4) 7.2 g/l, 34.9 g/l glucose, 20 g/l yeast extract, and 15 g/l acetate, which compared well to the predicated production of 12.13 g/l.
Related Concept Videos
Methods of Medium Optimization
Production of Organic Acids
Bioreactor Controls-III
Production of Antibiotics
Microbes in Food Production
Designing Growth Media for Bioreactors
