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Updated: Jun 23, 2026

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Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
Published on: February 8, 2014
Tomographic three-dimensional imaging of a biological specimen using wavelength-scanning digital interference
Optics Express
|May 2, 2009
Abstract:
The principle of wavelength-scanning digital interference holography is applied to three-dimensional imaging of a small biological specimen. The images are reconstructed from a number of holograms digitally recorded while the wavelengths are varied at regular intervals, and the numerical interference of the multiple three-dimensional hologram fields results in tomographic images with narrow axial resolution. An animated three-dimensional model of the object is constructed from the tomographic images.
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