Related Experiment Video
Updated: Jun 23, 2026

Establishment of a Clinically Relevant Ex Vivo Mock Cataract Surgery Model for Investigating Epithelial Wound Repair in a Native Microenvironment
Published on: June 5, 2015
[The effect of focal-adhesion micromanipulation in trauma and wound healing]
Yu-zhi Jiang1, Jun Wei, Bo Yuan
1Department of Burn, Burn Institute of Shanghai, Ruijin Hospital, Shanghai Jiaotong University, Shanghai, China.
Objective:
To explore the effect of focal-adhesion micromanipulation on the biological behavior of fibroblast.
Methods:
Micro-pot was made by microcontact printing. The molecules of constitutive protein was adhered on micro-pot by self-assemble of peptides. Skin fibroblasts were cultured on the membrane by self-made biomechanical cell culture for 2 weeks. Morphology observation and cell immunohistochemistry analysis was performed.
Results:
After 2 weeks, the morphology of the fibroblasts was diverse and more compliant. Cell immunohistochemistry analysis found that the expression of integrinbeta1, alpha5 and tensin was dramatically reduced.
Conclusions:
The morphology and the biological behaviour of the fibroblasts in hypertrophic scar can be changed after micromanipulation of focal adhesion.
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