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Related Experiment Video

Updated: Jun 23, 2026

Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs

Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs

Published on: December 19, 2019

Inflamed snail speeds metastasis.

Chih-Cheng Yang¹, Dieter A Wolf

¹Signal Transduction Program, Burnham Institute for Medical Research, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA.

|May 5, 2009

Summary

Tumor-promoting inflammation involves macrophages and cytokines. This study reveals how TNFalpha-NFkappaB signaling stabilizes Snail, driving cancer cell migration and metastasis.

Area of Science:

Oncology
Cell Biology
Molecular Biology

Background:

Macrophage infiltration and inflammatory cytokines are key drivers of cancer development and spread.
Understanding the molecular mechanisms linking inflammation to metastasis is crucial for therapeutic development.

Purpose of the Study:

To elucidate the molecular pathway through which inflammatory signals promote cancer cell migration and metastasis.
To identify key protein interactions and regulatory events involved in tumor progression.

Main Methods:

Investigated the role of tumor necrosis factor-alpha (TNFalpha) and nuclear factor-kappa B (NFkappaB) signaling.
Utilized biochemical assays to study the inhibition of glycogen synthase kinase 3 beta (GSK3beta) and SCF(beta-TRCP) ubiquitin ligase.
Analyzed the stabilization of the transcription factor Snail and its impact on cell migration.

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Last Updated: Jun 23, 2026

Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs

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Published on: December 19, 2019

Experimental Metastasis Assay

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Published on: August 24, 2010

CAM-Delam Assay to Score Metastatic Properties by Quantifying Delamination and Invasion Capacity of Cancer Cells

CAM-Delam Assay to Score Metastatic Properties by Quantifying Delamination and Invasion Capacity of Cancer Cells

Published on: June 2, 2022

Main Results:

Demonstrated that TNFalpha-dependent NFkappaB activation leads to COP9 signalosome-mediated inhibition of GSK3beta.
Showed that this inhibition prevents the ubiquitylation and degradation of the transcription factor Snail by the SCF(beta-TRCP) ubiquitin ligase.
Confirmed that Snail stabilization promotes cancer cell migration and metastasis.

Conclusions:

The inflammatory pathway involving TNFalpha and NFkappaB plays a critical role in promoting cancer metastasis through Snail stabilization.
Targeting this pathway could offer novel therapeutic strategies to inhibit tumor cell migration and prevent metastasis.