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Updated: Jun 23, 2026
A 3D Organotypic Melanoma Spheroid Skin Model
Published on: May 18, 2018
Characterization of phosphodiesterase 1 in human malignant melanoma cell lines
Kasumi Shimizu1, Taku Murata, Yoshihiro Watanabe
1Department of Oral and Maxillofacial Surgery, Mie University Graduate School of Medicine, 2-174 Edobashi, Japan. skasumi@clin.medic.mie-u.ac.jp
Background:
Differentiation-inducing factor 1 [DIF-1; 1-(3,5-dichloro-2,6-dihydroxy-4-methoxyphenyl) hexan-1-one] from Dictyostelium discoideum exhibits antiproliferative activity in mammalian cells. We have previously shown that phosphodiesterase 1 (PDE1) is a pharmacological target of DIF-1, but there are no reports of PDE1 in human malignant melanoma cells. Therefore, we characterized PDE1 in human malignant melanoma MAA cells.
Materials And Methods:
PDE1 mRNA expression was investigated in MAA cells. The full open reading frames for human PDE1C1 and PDE1C3 were cloned. Cell growth was determined by MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt] assay.
Results:
PDE1C mRNA expression was detected in MAA cells. The nucleotide sequence of PDE1C1 was identical to that of human PDE1C1, previously published. At nucleotide 2246 in PDE1C3, A was replaced by G, but this did not change the encoded amino acid. Cell growth was inhibited by the PDE1 inhibitor vinpocetin.
Conclusion:
PDE1C mRNA is expressed and may play an important role in human malignant melanoma MAA cells.
