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Updated: Jun 23, 2026
Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging
Published on: April 8, 2016
Immunohistochemical staining of mucin 1 in prostate tissues
Nadege Rabiau1, Pierre Dechelotte, Laurent Guy
1Département d'Oncogénétique du Centre Jean Perrin, Occupational Health Institute, CHU, 58 rue Montalembert, B.P.392, 63011 Clermont-Ferrand Cedex 01, France.
Unlabelled:
Prostate cancer is a major public health problem in the world. Molecular studies are necessary for the development of prognostic markers in prostate cancer. There is a great interest in mucin studies in treatment development of human malignancies, including prostate cancer. Nevertheless, their expressions in prostate cancer need further investigation.
Materials And Methods:
Mucin 1 (MUC1) expression was examined in 100 prostate biopsies and were compared with prostate carcinoma cell lines (DU-145, PC-3, LNCaP) by immunohistochemistry.
Results:
Biopsies were healthy, tumor, peritumor or presented an intraepithelial neoplasia. Staining of MUC1 was exihibited in PC-3 cells, was higher in DU-145, and was not expressed by LNCaP. Tumor sections presented more positive expression of MUC1 than non-tumor sections.
Conclusion:
MUC1 expression is correlated with the histological degree of malignancy.
