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Updated: Jun 23, 2026
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Fabrication of Amperometric Electrodes
Published on: May 4, 2009
Fabrication of amperometric electrodes
Carolyn M Pike1, Chad P Grabner, Amy B Harkins
1Department of Pharmacological and Physiological Science, Saint Louis University School of Medicine, USA.
Journal of Visualized Experiments : Jove
|May 6, 2009
Summary
This study details a refined protocol for fabricating low-noise carbon fiber electrodes essential for single-cell amperometry. These electrodes enable sensitive detection of catecholamine release, advancing neuroscience research.
Area of Science:
- Neuroscience
- Electrochemistry
- Cell Biology
Background:
- Carbon fiber electrodes are vital tools for detecting catecholamine release in single cells using amperometry.
- Achieving low-noise measurements (<0.5 pA) is critical for sensitive detection.
- Existing fabrication protocols require modification for consistent, high-quality electrode production.
Purpose of the Study:
- To describe a modified, detailed protocol for fabricating low-noise carbon fiber electrodes.
- To ensure consistent and damage-free production of electrodes for amperometric measurements.
- To provide a reliable method for preparing electrodes for single-cell recordings.
Main Methods:
- Preparation of carbon fibers and individual insertion into capillary tubes using a vacuum filtration system.
- Pulling capillary tubes with embedded fibers using an electrode puller to create fine-tipped electrodes.
- Sealing electrodes with a hot epoxy-glass mixture and oven curing for stability.
Main Results:
- Successfully fabricated carbon fiber electrodes with low noise levels (<0.5 pA).
- Demonstrated a reproducible protocol for consistent electrode production.
- Established a method for preparing electrodes suitable for single-cell amperometry.
Conclusions:
- The described protocol enables the reliable fabrication of low-noise carbon fiber electrodes.
- These electrodes are suitable for sensitive amperometric detection of catecholamine release from single cells.
- This method advances the capability for high-resolution electrophysiological recordings in cellular neuroscience.
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