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Updated: Jun 23, 2026

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence
Published on: March 9, 2015
Pigment phenotype and biogeographical ancestry from ancient skeletal remains: inferences from multiplexed autosomal
Caroline Bouakaze1, Christine Keyser, Eric Crubézy
1Laboratoire d'Anthropologie Moléculaire, Institut de Médecine Légale, 11 rue Humann, 67085 Strasbourg Cedex, France. caroline.bouakaze@ulp.u-strasbg.fr
Abstract:
In the present study, a multiplexed genotyping assay for ten single nucleotide polymorphisms (SNPs) located within six pigmentation candidate genes was developed on modern biological samples and applied to DNA retrieved from 25 archeological human remains from southern central Siberia dating from the Bronze and Iron Ages. SNP genotyping was successful for the majority of ancient samples and revealed that most probably had typical European pigment features, i.e., blue or green eye color, light hair color and skin type, and were likely of European individual ancestry. To our knowledge, this study reports for the first time the multiplexed typing of autosomal SNPs on aged and degraded DNA. By providing valuable information on pigment traits of an individual and allowing individual biogeographical ancestry estimation, autosomal SNP typing can improve ancient DNA studies and aid human identification in some forensic casework situations when used to complement conventional molecular markers.
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