Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying DNA...
Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the timing and level of...
Epigenetic Regulation01:37

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
Genomic Imprinting and Inheritance02:30

Genomic Imprinting and Inheritance

Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ZNF512B safeguards genome integrity at regulatory regions to repress the SASP and inflammation.

Cell stem cell·2026
Same author

Integrative Analysis Reveals Conserved R-Loop Features in Mouse Embryonic Stem Cells.

Epigenomes·2026
Same author

Distinctive DNA sequence features define epigenetic longevity of inflammatory memory.

Science (New York, N.Y.)·2026
Same author

The DNA methylation landscape of naturally short-lived killifish.

Scientific reports·2026
Same author

Non-disruptive in vitro monitoring of cellular states with cell-free DNA methylation.

Genome biology·2026
Same author

PD-1 blockade during T cell priming enhances long-term protection against metastatic tumors by epigenetically tuning T cell exhaustion.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: Jun 23, 2026

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
13:47

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Published on: March 29, 2019

Epigenetic hierarchy governing Nestin expression.

Dong Wook Han1, Jeong Tae Do, Marcos J Araúzo-Bravo

  • 1Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Münster, Germany.

Stem Cells (Dayton, Ohio)
|May 6, 2009
PubMed
Summary
This summary is machine-generated.

Histone acetylation, not DNA demethylation, activates Nestin gene expression in neural stem cells. This epigenetic mechanism is crucial for embryonic differentiation and can serve as a model for studying gene regulation.

More Related Videos

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis
10:25

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis

Published on: December 12, 2019

Related Experiment Videos

Last Updated: Jun 23, 2026

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
13:47

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Published on: March 29, 2019

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis
10:25

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis

Published on: December 12, 2019

Area of Science:

  • Neuroscience
  • Epigenetics
  • Developmental Biology

Background:

  • Nestin is a key intermediate filament protein in neural stem and progenitor cells.
  • Epigenetic modifications, including DNA methylation and histone modifications, regulate gene expression.
  • Understanding Nestin's epigenetic regulation is vital for neural development research.

Purpose of the Study:

  • To investigate the epigenetic regulation of Nestin gene expression during neural lineage differentiation.
  • To elucidate the roles of DNA demethylation and histone acetylation in Nestin gene activation.

Main Methods:

  • Utilized P19 embryonic carcinoma cells.
  • Applied epigenetic modifiers: trichostatin A (histone acetylation), 5-aza-2'-deoxycytidine (DNA demethylation), and retinoic acid (differentiation induction).
  • Analyzed DNA methylation and histone acetylation patterns in relation to Nestin expression.

Main Results:

  • Nestin expression showed differential DNA methylation and histone acetylation patterns.
  • Histone acetylation, induced by trichostatin A and retinoic acid, activated Nestin expression.
  • DNA demethylation did not appear to be the primary mechanism for Nestin activation in this model.

Conclusions:

  • Histone acetylation is sufficient to activate Nestin gene transcription during neural differentiation.
  • Nestin gene regulation serves as a model for studying histone acetylation-mediated gene expression, distinct from DNA demethylation.
  • Epigenetic control of Nestin is critical for neural cell lineage development.