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Related Experiment Video

Updated: Jun 23, 2026

Isolation of Sertoli Cells and Peritubular Cells from Rat Testes

Isolation of Sertoli Cells and Peritubular Cells from Rat Testes

Published on: February 8, 2016

Sertoli cell differentiation in pubertal boars.

J J Ford¹, T H Wise

¹USDA-ARS, US Meat Animal Research Center, Clay Center, NE 68933-0166, USA. joe.ford@ars.usda.gov

Journal of Animal Science

|May 8, 2009

Summary

Meishan boars reach puberty earlier than crossbred boars due to distinct Sertoli cell differentiation patterns. This study tracks anti-Mullerian hormone and CDKN1B expression, revealing a gradient of Sertoli cell maturation during boar pubertal development.

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Area of Science:

Reproductive Biology
Animal Science
Endocrinology

Background:

Meishan boars exhibit earlier puberty and smaller testes compared to crossbred boars.
Sertoli cell differentiation is crucial for pubertal development and testicular growth.

Purpose of the Study:

To define changes in anti-Mullerian hormone (AMH) and CDKN1B expression during prepubertal development in Meishan and crossbred boars.
To correlate these molecular changes with seminiferous tubule expansion and pubertal timing.

Main Methods:

Immunohistochemistry was used to assess AMH and CDKN1B expression in boar testes.
Temporal expression patterns were analyzed in relation to age and pubertal progression.
Tubular diameter and testis weight were measured.

Main Results:

AMH expression increased then decreased, followed by increased CDKN1B expression preceding tubule expansion in both breeds.
These pubertal events occurred earlier in Meishan boars.
Sertoli cell differentiation progressed as a gradient from the mediastinum outward.

Conclusions:

Temporal dynamics of AMH and CDKN1B mark Sertoli cell differentiation during boar puberty.
Breed-specific differences in these markers correlate with earlier pubertal onset in Meishan boars.
Sertoli cell differentiation occurs in a spatially organized manner within seminiferous tubules.