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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

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Related Experiment Video

Updated: Jun 23, 2026

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers
12:24

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

Published on: July 17, 2012

Large-image-format computed tomography imaging spectrometer for fluorescence microscopy.

B Ford, M Descour, R Lynch

    Optics Express
    |May 9, 2009
    PubMed
    Summary
    This summary is machine-generated.

    A new non-scanning multispectral imaging instrument captures full spectral data from living specimens in vivo. This advance enables real-time analysis of cellular function and physiological responses.

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    A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections
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    A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections

    Published on: March 30, 2020

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    Last Updated: Jun 23, 2026

    Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers
    12:24

    Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

    Published on: July 17, 2012

    A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections
    07:50

    A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections

    Published on: March 30, 2020

    Area of Science:

    • Biomedical Optics
    • Cellular Imaging
    • In Vivo Microscopy

    Background:

    • Multispectral imaging (MSI) excels in analyzing fixed pathology and cytogenetics specimens.
    • Current MSI applications in vivo are restricted by the need for high temporal resolution to study dynamic cellular processes.

    Purpose of the Study:

    • To develop a non-scanning instrument for in vivo multispectral imaging.
    • To enable simultaneous acquisition of full spectral data from every pixel in a 2-D field of view.

    Main Methods:

    • A novel non-scanning instrument was designed for rapid, simultaneous spectral data acquisition.
    • The system captures full spectral information (460-740 nm) within a single integration time (2 seconds).
    • Utilizes a 2-D field of view (200 µm x 200 µm) with spatial (0.985 µm) and spectral (5 nm) sampling.

    Main Results:

    • The instrument achieves high temporal resolution, capturing spectral data from all pixels simultaneously.
    • Demonstrates feasibility for analyzing dynamic physiological responses in living biological specimens.
    • Provides detailed spectral information at the pixel level for in vivo samples.

    Conclusions:

    • This non-scanning multispectral imaging approach overcomes limitations of previous in vivo applications.
    • The technology facilitates the study of cellular function and physiological changes in real-time.
    • Opens new avenues for in vivo pathological and cytogenetic analysis.