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Updated: Jun 23, 2026

Isolation and Time-Lapse Imaging of Primary Mouse Embryonic Palatal Mesenchyme Cells to Analyze Collective Movement Attributes
Published on: February 13, 2021
MOTION FLOW ESTIMATION FROM IMAGE SEQUENCES WITH APPLICATIONS TO BIOLOGICAL GROWTH AND MOTILITY
Gang Dong1, Tobias I Baskin, Kannappan Palaniappan
1Department of Biology, University of Massachusetts, Amherst, Massachusetts 01003 USA.
Abstract:
In this paper, a new method for motion flow estimation that considers errors in all the derivative measurements is presented. Based on the total least squares (TLS) model, we accurately estimate the motion flow in the general noise case by combining noise model (in form of covariance matrix) with a parametric motion model. The proposed algorithm is tested on two different types of biological motion, a growing plant root and a gastrulating embryo, with sequences obtained microscopically. The local, instantaneous velocity field estimated by the algorithm reveals the behavior of the underlying cellular elements.

