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Related Experiment Video

Updated: Jun 23, 2026

Characterizing Single-Molecule Conformational Changes Under Shear Flow with Fluorescence Microscopy

Characterizing Single-Molecule Conformational Changes Under Shear Flow with Fluorescence Microscopy

Published on: January 25, 2020

Microrheology with fluorescence correlation spectroscopy.

Silke Rathgeber¹, Hans-Josef Beauvisage, Hubert Chevreau

¹Max Planck-Institute for Polymer Research, Polymer Physics, 55128 Mainz, Germany. s.rathgeber@mpip-mainz.mpg.de

Langmuir : the ACS Journal of Surfaces and Colloids

|May 12, 2009

Summary

This summary is machine-generated.

Fluorescence correlation spectroscopy (FCS) enables passive microrheology (MR) measurements, revealing local material properties. This technique accurately characterizes rheological behavior across a wide frequency range, showing good agreement with conventional methods.

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Combining Microfluidics and Microrheology to Determine Rheological Properties of Soft Matter during Repeated Phase Transitions

Combining Microfluidics and Microrheology to Determine Rheological Properties of Soft Matter during Repeated Phase Transitions

Published on: April 19, 2018

Area of Science:

Materials Science
Physical Chemistry
Biophysics

Background:

Conventional rheological methods often require large sample volumes and may not capture local variations.
Passive microrheology (MR) offers a way to probe material properties on smaller length scales.
Fluorescence correlation spectroscopy (FCS) is a sensitive technique for studying molecular dynamics.

Purpose of the Study:

To demonstrate the application of fluorescence correlation spectroscopy (FCS) for passive microrheological (MR) measurements.
To validate FCS-based MR by comparing results with conventional rheology techniques.
To explore the potential of FCS-MR for probing length-scale-dependent rheological properties in heterogeneous materials.

Main Methods:

Utilized a commercial spectrometer for fluorescence correlation spectroscopy (FCS) measurements.
Performed experiments on a high molecular weight poly(ethylene oxide)-water solution.
Compared FCS-derived mean-square displacements and shear moduli with data from diffusing wave spectroscopy, quasielastic light scattering, particle tracking, rotational rheometry, and squeeze flow apparatus.

Main Results:

Successfully detected mean-square displacements from 100 nm² to 10⁶ nm².
Derived bulk rheological shear moduli spanning over five decades of frequency (10⁻¹ to 10⁴ rad/s).
Achieved good agreement between FCS-MR results and conventional rheological measurements across the entire frequency range.

Conclusions:

Fluorescence correlation spectroscopy (FCS) is a viable and powerful tool for passive microrheology (MR).
FCS-MR provides accurate local rheological property measurements that correlate well with macroscopic behavior.
The spatial resolution and sensitivity of FCS-MR make it particularly suitable for heterogeneous samples, including biological and medical applications.