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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

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Related Experiment Video

Updated: Jun 23, 2026

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

Novel application for isothermal nucleic acid sequence-based amplification (NASBA).

Anne Böhmer1, Verena Schildgen, Jessica Lüsebrink

  • 1Institute of Virology, University of Bonn Medical Centre, Bonn, Germany.

Journal of Virological Methods
|May 12, 2009
PubMed
Summary
This summary is machine-generated.

Human bocavirus (HBoV) research is advanced by a new nucleic acid sequence-based amplification (NASBA) method. This technique confirms HBoV

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Nanopore DNA Sequencing for Metagenomic Soil Analysis
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Nanopore DNA Sequencing for Metagenomic Soil Analysis

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Related Experiment Videos

Last Updated: Jun 23, 2026

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings
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Nanopore DNA Sequencing for Metagenomic Soil Analysis
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Nanopore DNA Sequencing for Metagenomic Soil Analysis

Published on: December 14, 2017

Area of Science:

  • Virology
  • Molecular Biology
  • Genomics

Background:

  • Human bocavirus (HBoV) is a human parvovirus.
  • Lack of cell cultures and animal models hinders HBoV research.
  • Understanding HBoV genome structure and replication is challenging.

Purpose of the Study:

  • To investigate the nature of the HBoV genome packaged within virions.
  • To evaluate the utility of nucleic acid sequence-based amplification (NASBA) for HBoV detection and characterization.
  • To confirm the single-stranded negative-sense nature of HBoV genomes.

Main Methods:

  • Nucleic acid sequence-based amplification (NASBA) was applied to HBoV samples.
  • Analysis focused on detecting positive and negative strands of the HBoV genome.
  • Phylogenetic analyses were previously used for HBoV classification.

Main Results:

  • NASBA detected the negative strand of the HBoV genome in 100% of positive samples.
  • A detectable amount of the positive HBoV genome strand was found in 14.3% of samples.
  • The single-stranded negative-sense nature of HBoV genomes was confirmed across subtypes.

Conclusions:

  • NASBA is a valuable tool for HBoV diagnosis and research.
  • The study confirms the negative-sense, single-stranded genome of Human bocavirus.
  • This finding aids in understanding HBoV replication and viral structure.