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Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
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Fluorescence detection of single-nucleotide polymorphisms with a single, self-complementary, triple-stem DNA probe

Yi Xiao1, Kory J I Plakos, Xinhui Lou

  • 1Materials Department, University of California, Santa Barbara, Santa Barbara, CA 93106, USA.

Angewandte Chemie (International Ed. in English)
|May 12, 2009
PubMed
Summary

This study introduces a novel, single-step DNA sensor for detecting single-nucleotide polymorphisms (SNPs) at room temperature. The innovative sensor utilizes a unique DNA structure that significantly enhances fluorescence upon binding to target sequences.

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