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Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
Published on: January 5, 2021
Multidimensional quantification of subcellular morphology of Saccharomyces cerevisiae using CalMorph, the
Takahiro Negishi1, Satoru Nogami, Yoshikazu Ohya
1Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa-shi, Chiba, Japan.
Journal of Biotechnology
|May 13, 2009
Summary
Researchers quantitatively assessed budding yeast cell morphology using the CalMorph program. This method analyzes 1111 parameters for subcellular components, advancing yeast functional genomics studies.
Area of Science:
- Cell Biology
- Genomics
- Biophysics
Background:
- Quantitative assessment of subcellular morphology is crucial for understanding cellular function.
- Existing methods may lack the multidimensionality required for comprehensive analysis.
- Budding yeast is a model organism for studying fundamental cellular processes.
Purpose of the Study:
- To present CalMorph, an image-processing program for quantitative, multidimensional morphological assessment of subcellular components in budding yeast.
- To expand the parameter set for comprehensive morphological evaluation.
- To facilitate detailed phenotypic studies in yeast functional genomics.
Main Methods:
- Application of the CalMorph image-processing program to budding yeast cells.
- Evaluation of morphology for mitochondria, vacuole, endoplasmic reticulum, Golgi body, endosome, spindle pole body, and septin.
- Expansion of morphological parameters from 501 to a total of 1111, including those for cell wall, nuclear DNA, and actin.
Main Results:
- CalMorph enables quantitative and multidimensional assessment of diverse subcellular organelles and protein complexes.
- A total of 1111 quantitative parameters were utilized for comprehensive yeast morphology evaluation.
- The expanded parameter set allows for more detailed phenotypic characterization.
Conclusions:
- The CalMorph program provides a powerful tool for detailed morphological studies in budding yeast.
- This approach significantly enhances the capacity for phenotypic analysis in yeast functional genomics.
- Multidimensional quantitative morphology assessment is key to advancing our understanding of cellular mechanisms.
