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Related Experiment Video

Updated: Jun 23, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Next generation chemical proteomic tools for rapid enzyme profiling.

Mahesh Uttamchandani¹, Candy H S Lu, Shao Q Yao

¹Defence Medical and Environmental Research Institute, DSO National Laboratories, 27 Medical Drive, Singapore 117510.

Accounts of Chemical Research

|May 14, 2009

Summary

Enzyme characterization is crucial for understanding life and developing new drugs. New high-throughput technologies enable rapid profiling and discovery of small molecules to modulate enzyme activity.

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Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Published on: August 8, 2016

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Last Updated: Jun 23, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Published on: August 8, 2016

Area of Science:

Biochemistry
Chemical Biology

Background:

Enzymes are essential catalysts for cellular processes, regulating life, and are key targets for drug development.
Despite their importance, the roles and specificities of most enzymes remain largely uncharacterized.
Understanding enzyme interactions is vital for biological insights and therapeutic strategies.

Purpose of the Study:

To present technological advances for high-throughput enzyme study and characterization.
To bridge the knowledge gap regarding the physiological roles and substrate specificities of uncharacterized enzymes.
To facilitate the discovery of novel enzyme inhibitors for therapeutic applications.

Main Methods:

Development of enabling platforms and chemical tools, including activity-based probes and compound libraries.
Utilizing combinatorial synthesis and click chemistry for rapid library assembly.
High-throughput screening of molecular assortments against target enzymes using microplates or microarrays.

Main Results:

Demonstrated technological advances for studying enzymes and their properties in a high-throughput manner.
Successfully developed chemical probes and libraries for comparative enzyme screening.
Enabled rapid profiling and characterization of various enzyme classes, including kinases, phosphatases, and proteases.

Conclusions:

High-throughput technologies offer powerful means to study, profile, and discover small molecules that modulate enzyme activity.
These advancements are transforming the rapid profiling and characterization of enzymes.
The developed chemical tools and screening platforms are crucial for enzyme research and drug discovery.