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This protocol presents an approach for whole transcriptome analysis from zebrafish embryos, larvae, or sorted cells. We include isolation of RNA, pathway analysis of RNASeq data, and qRT-PCR-based validation of gene expression...

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Immunohistochemistry is a powerful lab technique for evaluating protein localization and expression within tissues. Current semi-automated methods for quantitation introduce subjectivity and often create irreproducible results. Herein, we describe methods for multiplexed immunohistochemistry and objective quantitation of protein expression and co-localization using multispectral...

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Related Experiment Video

Updated: Jan 20, 2026

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Published on: October 27, 2017

[How to express quantitative data: part four]

Liang-ping Hu¹, Hui-gang Liu, Li-xin Tao

¹Consulting Center of Biomedical Statistics, Academy of Military Medical Sciences, Beijing 100850, China. lphu812@sina.com

Zhong Xi Yi Jie He Xue Bao = Journal of Chinese Integrative Medicine

|May 14, 2009

Summary

No abstract available in PubMed .

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Published on: October 25, 2011

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Last Updated: Jan 20, 2026

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Published on: October 27, 2017

Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps

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Published on: February 9, 2017

Heterogeneity Mapping of Protein Expression in Tumors using Quantitative Immunofluorescence

Heterogeneity Mapping of Protein Expression in Tumors using Quantitative Immunofluorescence

Published on: October 25, 2011