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DNA Microarrays

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Related Experiment Video

Updated: Jun 23, 2026

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

Intra-platform repeatability and inter-platform comparability of microRNA microarray technology.

Fumiaki Sato¹, Soken Tsuchiya, Kazuya Terasawa

¹Department of Nanobio Drug Discovery, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Kyoto, Japan. fsato@pharm.kyoto-u.ac.jp

|May 14, 2009

Summary

This summary is machine-generated.

This study compared five microRNA microarray platforms, finding Agilent and Toray arrays performed best. While intra-platform repeatability was high, inter-platform concordance was poor due to normalization and detection criteria issues.

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Last Updated: Jun 23, 2026

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Area of Science:

Life Sciences
Molecular Biology
Genomics

Background:

DNA microarray technology has significantly advanced life sciences.
The MicroArray Quality Control (MAQC) project established standards for microarray analysis.
Microarray technology is increasingly used for comprehensive microRNA expression profiling.

Purpose of the Study:

To compare the repeatability and comparability of five commercial microRNA microarray platforms.
To evaluate microRNA microarray performance against the Taqman microRNA system.
To identify limitations in current microRNA microarray systems.

Main Methods:

Comparative analysis of five microRNA microarray platforms (Agilent, Ambion, Exiqon, Invitrogen, Toray) using 309 microRNA probes.
Validation against the Taqman microRNA system with 142 microRNA probes.
Assessment of intra-platform repeatability and inter-platform comparability.

Main Results:

MicroRNA microarrays demonstrated high intra-platform repeatability and comparability with quantitative RT-PCR.
Agilent and Toray platforms exhibited superior performance among the tested microRNA microarray systems.
Significant lack of inter-platform concordance was observed, attributed to inadequate normalization and varied detection criteria.

Conclusions:

MicroRNA microarrays offer reliable intra-platform results but lack standardization for inter-platform comparisons.
Agilent and Toray platforms are recommended for microRNA expression profiling based on performance.
Further development in normalization methods and detection criteria is crucial for improving microRNA microarray systems.