Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Newt satellite 2 transcripts self-cleave by using an extended hammerhead structure.

L M Pabón-Peña1, Y Zhang, L M Epstein

  • 1Department of Biological Science, Florida State University, Tallahassee 32306-3050.

Molecular and Cellular Biology
|December 1, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Two pathways of proton transfer reaction to (triphos)Cu(η(1)-BH4) via a dihydrogen bond [triphos = 1,1,1-tris(diphenylphosphinomethyl)ethane].

Dalton transactions (Cambridge, England : 2003)·2016
Same author

Use of intracardiac echocardiography to guide ablation of atrial fibrillation.

Circulation·2001
Same author

Crown compounds for anions: sandwich and half-sandwich complexes of cyclic trimetric perfluoro-o-phenylenemercury with polyhedral closo-[B10H10]2- and closo-[B12H12]2- anions.

Chemistry (Weinheim an der Bergstrasse, Germany)·2001
Same author

Recalls and safety alerts involving pacemakers and implantable cardioverter-defibrillator generators.

JAMA·2001
Same author

SSeCKS gene expression in vascular smooth muscle cells: regulation by angiotensin II and a potential role in the regulation of PAI-1 gene expression.

Journal of molecular and cellular cardiology·2000
Same author

A novel indication for transvenous lead extraction: upgrading implantable cardioverter defibrillator systems.

Journal of interventional cardiac electrophysiology : an international journal of arrhythmias and pacing·2000

Newt satellite 2 DNA self-cleaves via a hammerhead-like domain, but requires flanking sequences for activity. A structural model reveals an internal loop extension critical for this essential self-cleavage mechanism.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • Satellite 2 DNA from newts exhibits self-catalyzed, site-specific cleavage in vitro.
  • The cleavage domain resembles the hammerhead ribozyme found in plant RNAs.
  • The newt hammerhead structure is potentially unstable, unlike previously studied hammerheads.

Purpose of the Study:

  • To investigate the requirements for self-cleavage of newt satellite 2 DNA.
  • To elucidate the structural basis for the cleavage activity.
  • To identify key nucleotides involved in the catalytic process.

Main Methods:

  • In vitro self-cleavage assays using synthetic satellite 2 DNA transcripts.
  • In vitro mutagenesis to alter specific nucleotides within the hammerhead domain and flanking regions.

Related Experiment Videos

  • Structural modeling of a truncated, active satellite 2 transcript.

    • Main Results:

    • Cleavage requires satellite 2 sequences flanking the hammerhead domain; it does not cleave in isolation.
    • A structural model shows an internally looped extension to a conserved stem, crucial for activity.
    • Mutagenesis confirmed that five nucleotides in an internal loop are critical for cleavage.
    • Evidence suggests the extension stimulates cleavage beyond simple stabilization.

    Conclusions:

    • Newt satellite 2 DNA self-cleavage is dependent on flanking sequences and a specific structural motif.
    • The internally looped extension plays a critical role in enhancing catalytic activity.
    • This study provides insights into the mechanism of hammerhead-like ribozymes in a non-plant system.