Related Experiment Video
Updated: Jun 23, 2026
A Simple Flow Cytometry Based Assay to Determine In Vitro Antibody Dependent Enhancement of Dengue Virus Using Zika Virus Convalescent Serum
Published on: April 10, 2018
Immunoglobulin G antibody response in children and adults with acute dengue 3 infection
Susana Vazquez1, Nadia Acosta, Didye Ruiz
1Pedro Kourí Tropical Medicine Institute (IPK), PAHO/WHO Collaborating Center for the Study of Dengue and its Vector, Havana City, Cuba. svazquez@ipk.sld.cu
Insights
This study defines criteria for primary or secondary dengue virus infection using IgG antibody titers. Findings differentiate infection types in children versus adults during Cuba's 2001-2002 dengue epidemic.
Area of Science:
- Virology
- Immunology
- Epidemiology
Background:
- Dengue virus infection classification relies on serological tests.
- Cuba faced a significant dengue epidemic in 2001-2002.
Purpose of the Study:
- To evaluate IgG antibody response for classifying dengue virus infection.
- To differentiate primary and secondary dengue infections in acute-phase samples.
Main Methods:
- ELISA inhibition assay used on 713 serum samples from IgM-positive patients.
- Samples collected 5-7 days post-fever onset from children and adults.
- Control samples from healthy donors and non-dengue patients.
Main Results:
- Children showed a primary IgG infection pattern (89.3% with titers ≤20).
- Adults exhibited both primary (13.4% with titers ≤20) and secondary (83.9% with titers ≥1280) patterns.
- Established criteria for classifying dengue infection based on IgG titers.
Conclusions:
- Defined serological criteria for primary/secondary dengue virus infection.
- Distinguished infection patterns between pediatric and adult populations.
- Facilitated accurate dengue case classification during acute phases.
Abstract:
Using a serological test, different criteria have been established for classifying a case as primary or secondary dengue virus infection. Considering the dengue epidemiological situation in Cuba, IgG antibody response to dengue virus infection in serum samples from children and adults with a dengue 3 infection, in Havana city during the 2001-2002 epidemic was evaluated. Samples were collected on days 5-7 of fever onset and tested by an ELISA inhibition. A total of 713 serum samples positive for IgM antibody, 93 from children and 620 from adult patients were studied. Serum samples collected from healthy blood donors and patients not infected with dengue were included as controls. An IgG primary infection pattern was observed in sera collected from children, with titers of < or =20 in the 89.3% of the patients, while both, a primary and secondary patterns were observed in sera collected from adult patients with titers of < or =20 (13.4%) and > or =1280 (83.9%), respectively. These results permitted the definition of a primary or secondary case of dengue virus infection in serum samples collected during the acute phase of dengue virus infection.
More Related Videos
06:34An Optimized Hemagglutination Inhibition (HI) Assay to Quantify Influenza-specific Antibody Titers
Published on: December 1, 2017
06:00Portable Paper-Based Immunoassay Combined with Smartphone Application for Colorimetric and Quantitative Detection of Dengue NS1 Antigen
Published on: January 26, 2024
Related Concept Videos
01:22Development of Immunocompetence
The initial cells that migrate from the fetal thymus settle within the skin and epithelial tissues lining the mouth, digestive tract, and in females, the uterus and vagina. These cells, including skin-based dendritic cells, serve as antigen-presenting cells, playing a key role in T cell activation.
Subsequent T...
01:23Immunological Memory
What is Immunological Memory?
Immunological memory is an integral function of the immune system that allows it to recognize and react more rapidly and effectively to pathogens previously encountered. This feature is...
00:42Cross-reactivity