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Related Experiment Video

Updated: Jun 23, 2026

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
11:40

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

Microfluidic-assisted analysis of replicating DNA molecules.

Julia M Sidorova1, Nianzhen Li, David C Schwartz

  • 1Department of Pathology, University of Washington, Seattle, Washington, USA. julias@u.washington.edu

Nature Protocols
|May 16, 2009
PubMed
Summary

This study introduces a novel microfluidic platform for stretching and capturing DNA molecules. This innovation simplifies DNA replication analysis from small biological samples.

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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Microfluidics

Background:

  • Single-molecule techniques are crucial for visualizing DNA replication.
  • Current methods often require larger sample volumes and complex procedures.
  • Incorporating nucleoside analogs aids in DNA replication studies.

Purpose of the Study:

  • To develop a microfluidic platform for efficient DNA stretching and capture.
  • To enable high-resolution DNA replication analysis from minimal samples.
  • To simplify and enhance existing single-molecule DNA replication protocols.

Main Methods:

  • Fabrication of polydimethylsiloxane microchannels using soft lithography.
  • Utilizing capillary tension within microchannels for DNA manipulation.

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Visualizing Single-molecule DNA Replication with Fluorescence Microscopy
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Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

Published on: October 9, 2009

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

Related Experiment Videos

Last Updated: Jun 23, 2026

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
11:40

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy
15:57

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

Published on: October 9, 2009

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

  • Employing silane-modified glass coverslips for reversible sealing and DNA deposition.

    • Main Results:

    • Demonstrated a microfluidic platform capable of stretching and capturing labeled DNA molecules.
    • Successfully utilized capillary tension for DNA loading, stretching, and deposition.
    • Platform accommodates microliter-scale DNA samples.

    Conclusions:

    • The described microfluidic platform offers a simple and extensible method for DNA replication analysis.
    • This technology facilitates replication studies using small biological and clinical samples.
    • The platform has the potential to advance genomic and locus-specific DNA replication research.