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Updated: Jun 23, 2026

In Vivo Multimodal Imaging and Analysis of Mouse Laser-Induced Choroidal Neovascularization Model
Published on: January 21, 2018
Fusion of images in multistep analysis of neovascularisation
Sergey V Tokalov1, Andrij M Abramyuk
1OncoRay (Center for Radiation Research in Oncology), University of Technology-Dresden, Dresden, Germany. sergey.tokalov@oncoray.de
Purpose:
The aim of our study was to develop a method for the fusion of images received after repeated staining of the same sample taking into account spatial differences between the images.
Material And Methods:
A method of objective fusion performance was investigated on the images receiving during multistep staining of the xenograft tumour cross-sections.
Results:
It was shown that several images receiving from different steps of staining procedures may be successfully fused by fluorescent marking of slide position with Trout red blood cells before analysis.
Conclusions:
Proposed technique provides an accurate rigid fusion of light and fluorescent images receiving during multistep image analysis under microscope and may be applied for study of neovascularisation.
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