[Study on experiment of fluorescence spectra detection of organic pesticides in soil]

The fluorescence characteristics of carbamate pesticide, namely carbaryl, was studied based on the basic theory that organic molecules can emit fluorescence as they are excited by rays. Consequently, a fluorescence spectrograph was applied to conduct fluorescence spectrum experiments with standard solution of carbaryl and the hydrolyzed carbaryl, the fluorescence spectra were obtained under the condition of different concentration, and the relation between their fluorescence intensity and concentration was also analyzed. On the basis of this, using the soil as a medium, the fluorescence characteristics of carbaryl and the hydrolyzed carbaryl in the soil were researched as well. The experiment results show that carbaryl and the hydrolyzed carbaryl in the soil can also emit intensive fluorescence which is similar to that in solution as being excited by UV rays. The fluorescence spectra are located between 400 and 750 nm and they all have smooth spectrum forms and fine resolution, so the spectra are suitable for qualitative and quantitative analysis of carbaryl. As a result, it is feasible to carry out the detection and analysis of the concerned pesticides in soil directly or indirectly by fluorescence spectral analysis.