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Updated: Jun 23, 2026

Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos
Published on: October 9, 2020
The early secretory pathway in development: a tale of proteins and mRNAs
Vangelis Kondylis1, Sandrine Pizette, Catherine Rabouille
1Cell Microscopy Centre, Department of Cell Biology, UMC Utrecht, Utrecht, The Netherlands.
Abstract:
The secretory pathway ensures the proper delivery of secreted proteins to the extracellular medium and of transmembrane proteins to almost all membrane cellular compartments. During their transport in the different compartments making up this pathway, newly synthesized proteins are modified and dispatched to their final destinations. So far, this pathway has mostly been studied in tissue cultured cells or yeast but recently, mutations in genes encoding key proteins of this pathway have been shown to lead to severe developmental defects in different model organisms. In this review, we describe how specific steps of epithelial, cartilage, notochord and brain development as well as body axis formation are controlled by the early secretory machinery illustrating that it is as crucial as transcriptional programs.
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