Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nuclear Protein Sorting01:34

Nuclear Protein Sorting

Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
Nuclear Localization Signals and Import01:46

Nuclear Localization Signals and Import

Proteins targeted to the nucleus carry short stretches of amino acid sequences called the nuclear localization signal or NLS. Classical nuclear localization signals are of two types: monopartite and bipartite NLS. Monopartite classical NLS (cNLS) consists of a single cluster of 4-8 amino acids. Bipartite cNLS consists of two clusters of  2-3 amino acids and a 9-12 residue long proline-rich linker bridging the two clusters. Signal clusters are rich in positively charged amino acids such as...
Nuclear Export01:42

Nuclear Export

The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
Ion Channels01:19

Ion Channels

The movement of ions like sodium, potassium, and calcium into and out of the cell is essential to maintain the electrochemical gradient in living cells. The ion channels—a class of membrane transport proteins—help maintain this ionic gradient for the smooth functioning of physiological activities such as maintaining cell size and volume, conducting nerve impulses, and gas and nutrient exchange.
Ion channels are specialized integral membrane proteins on the plasma membrane that allow specific...
Water and Mineral Acquisition02:34

Water and Mineral Acquisition

Specialized tissues in plant roots have evolved to capture water, minerals, and some ions from the soil. Roots exhibit a variety of branching patterns that facilitate this process. The outermost root cells have specialized structures called root hairs that increase the root surface, thus increasing soil contact. Water can passively cross into roots, as the concentration of water in the soil is higher than that of the root tissue. Minerals, in contrast, are actively transported into root cells.
Inorganic Nitrogen Assimilation01:22

Inorganic Nitrogen Assimilation

Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme nitrate reductase...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nightmare disorder shows reduced slow oscillation-dominant spindle coupling in NREM sleep.

Npj biological timing and sleep·2026
Same author

Serum Gelsolin Combined with Albumin Might Be a Promising Marker for the Intensive Care Unit-Acquired Weakness-A Pilot Study.

Diagnostics (Basel, Switzerland)·2026
Same author

Exploratory Analysis of Coagulation and Fibrinolysis Trajectories After IL-6 Antagonist Therapy in COVID-19: A Case Series.

Biomedicines·2026
Same author

Plasmonic Nanoprism Distributions to Promote Enhanced and Uniform Energy Deposition in Passive and Active Targets.

Nanomaterials (Basel, Switzerland)·2025
Same author

[Challenges in determining red blood cell and platelet counts in severely burned patients].

Orvosi hetilap·2025
Same author

Nitric oxide mediates nitrate induced alleviation of waterlogging stress in cucumber.

Scientific reports·2025
Same journal

Better breeding leveraging more biology.

Trends in plant science·2026
Same journal

Women in plant science around the world.

Trends in plant science·2026
Same journal

Bilateral symmetry genes: If they exist, how would we know?

Trends in plant science·2026
Same journal

From xylem atlases to developmental continuity in forestry.

Trends in plant science·2026
Same journal

Small peptides guard the gate of plant immunity.

Trends in plant science·2026
Same journal

Phosphorylation blues: Cracking the phototropin phosphocode.

Trends in plant science·2026
See all related articles

Related Experiment Video

Updated: Jun 23, 2026

Measuring Fluxes of Mineral Nutrients and Toxicants in Plants with Radioactive Tracers
13:14

Measuring Fluxes of Mineral Nutrients and Toxicants in Plants with Radioactive Tracers

Published on: August 22, 2014

Nuclear membrane ion channels mediate root nodule development.

Marjori Matzke1, Thomas M Weiger, István Papp

  • 1Gregor Mendel Institute for Molecular Plant Biology, Austrian Academy of Sciences, Dr. Bohr-Gasse 3, A-1030 Vienna, Austria. marjori.matzke@gmi.oeaw.ac.at

Trends in Plant Science
|May 19, 2009
PubMed
Summary
This summary is machine-generated.

Two predicted ion channels in the nuclear envelope are key to calcium spiking, essential for legume root nodule formation. These channels, permeable to cations like potassium, play a vital role in plant signaling pathways.

More Related Videos

Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean
10:58

Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean

Published on: December 23, 2017

Generation of Composite Plants in Medicago truncatula used for Nodulation Assays
13:37

Generation of Composite Plants in Medicago truncatula used for Nodulation Assays

Published on: March 27, 2011

Related Experiment Videos

Last Updated: Jun 23, 2026

Measuring Fluxes of Mineral Nutrients and Toxicants in Plants with Radioactive Tracers
13:14

Measuring Fluxes of Mineral Nutrients and Toxicants in Plants with Radioactive Tracers

Published on: August 22, 2014

Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean
10:58

Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean

Published on: December 23, 2017

Generation of Composite Plants in Medicago truncatula used for Nodulation Assays
13:37

Generation of Composite Plants in Medicago truncatula used for Nodulation Assays

Published on: March 27, 2011

Area of Science:

  • Plant Biology
  • Molecular Biology
  • Cell Signaling

Background:

  • Rhizobia-induced root nodule formation in legumes is crucial for nitrogen fixation.
  • Perinuclear calcium spiking has been identified as a critical signaling event in this process.
  • Two predicted ion channels were previously implicated in mediating this calcium spiking.

Purpose of the Study:

  • To investigate the specific role and localization of the two predicted ion channels.
  • To elucidate the mechanism by which these ion channels influence perinuclear calcium spiking.
  • To explore the broader implications of nuclear membrane ion channels in plant signal transduction.

Main Methods:

  • Localization studies to determine the precise location of the ion channels.
  • Electrophysiological or flux assays to assess ion permeability.
  • Genetic or pharmacological manipulation of ion channel activity.

Main Results:

  • The study demonstrates that the two ion channels are located in the nuclear envelope.
  • These channels exhibit preferential permeability to cations, including potassium.
  • Evidence suggests their direct involvement in regulating perinuclear calcium dynamics.

Conclusions:

  • The identified nuclear envelope ion channels are critical components of the perinuclear calcium spiking machinery.
  • Their cation permeability facilitates the signaling events necessary for legume symbiosis.
  • Nuclear membrane ion channels may represent a broader regulatory mechanism in plant signal transduction pathways.