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Updated: Jun 23, 2026

Corneal Donor Tissue Preparation for Endothelial Keratoplasty
Published on: June 12, 2012
Corneal flap thickness with the Moria M2 microkeratome and Med-Logics calibrated LASIK blades
Juhani Pietilä1, Anne Huhtala, Petri Mäkinen
1Mehiläinen Eye Surgery Clinic, Tampere, Finland.
Purpose:
This study aimed to compare and study potential factors that affect the accuracy of corneal flap thickness created in laser-assisted in situ keratomileusis (LASIK) using the Moria model 2 (M2) head 130 microkeratome with the Med-Logics calibrated LASIK blades Minus 20 (ML -20) and Minus 30 (ML -30).
Methods:
Corneal thickness in 200 (164 myopic and 36 hyperopic) eyes (100 patients) was measured by ultrasonic pachymetry preoperatively and intraoperatively after flap cutting. A total of 100 eyes were treated with the ML -20 and 100 with the ML -30. The right eye was operated before the left eye in each patient, using the same blade. In an additional group of 40 eyes, the left eye was operated first.
Results:
Mean corneal flap thickness using the ML -20 blade for an intended flap thickness of 140 μm was 129.1 μm (standard deviation [SD] 15.6, range 104-165 μm) in right eyes and 111.5 μm (SD 14.5, range 78-144 μm) in left eyes. Mean corneal flap thickness using the ML -30 blade for an intended flap thickness of 130 μm was 127.1 μm (SD 16.6, range 90-168 μm) in right eyes and 109.9 μm (SD 16.8, range 72-149 μm) in left eyes.
Conclusions:
Both microkeratome blade types cut thinner flaps than were intended. There was substantial variation in flap thickness. The first flap to be cut with a particular blade was considerably thicker than the second flap cut with the same blade. Based on these data, we recommend the use of disposable single-use microkeratomes rather than these ML blades.

