Related Experiment Video
Updated: Jun 23, 2026
08:37
Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
Published on: March 30, 2015
Development of a pUC19-based recombinant plasmid to serve as a positive control in PCR for Orientia tsutsugamushi
Nozomu Hanaoka1, Akiko Sakata, Ai Takano
1Department of Virology-I, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo 162-8640, Japan.
Microbiology and Immunology
|May 22, 2009
Abstract:
A recombinant positive control plasmid was developed to be used in PCR for Orientia tsutsugamushi. This pUC19-based plasmid contains the O. tsutsugamushi DNA sequence, part of which is replaced with Candida albicans DNA. pUC19-Posi is a PCR control that provides several advantages over currently used positive controls for both universal and serotypical PCR.
