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Related Concept Videos

Computed Tomography01:10

Computed Tomography

Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Imaging Studies III: Computed Tomography01:27

Imaging Studies III: Computed Tomography

DefinitionComputed Tomography (CT) of the genitourinary (GU) tract is a non-invasive imaging modality that utilizes X-rays and computer processing to generate detailed cross-sectional images of the urinary system, encompassing the kidneys, ureters, bladder, and adjacent structures such as the adrenal glands.PurposeCT scans of the GU tract serve several diagnostic and therapeutic purposes, including:Diagnosis of Urinary Tract Diseases: Detects kidney stones, tumors, cysts, and congenital...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Positron Emission Tomography01:29

Positron Emission Tomography

Positron emission tomography (PET) is a medical imaging technique involving radiopharmaceuticals — substances that emit short-lived radiation. Although the first PET scanner was introduced in 1961, it took 15 more years before radiopharmaceuticals were combined with the technique and revolutionized its potential.
One of the main requirements of a PET scan is a positron-emitting radioisotope, which is produced in a cyclotron and then attached to a substance used by the part of the body being...

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Related Experiment Video

Updated: Jun 23, 2026

Doppler Optical Coherence Tomography of Retinal Circulation
10:46

Doppler Optical Coherence Tomography of Retinal Circulation

Published on: September 18, 2012

Real-time multi-functional optical coherence tomography.

Boris Park, Mark Pierce, Barry Cense

    Optics Express
    |May 23, 2009
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a multi-functional optical coherence tomography (MF-OCT) system for real-time imaging of tissue structure, birefringence, and blood flow. The efficient data processing enables high-speed, detailed visualization without hardware modifications.

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    Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
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    Last Updated: Jun 23, 2026

    Doppler Optical Coherence Tomography of Retinal Circulation
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    Published on: September 18, 2012

    Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
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    Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
    11:21

    Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

    Published on: January 15, 2013

    Area of Science:

    • Biomedical Optics
    • Medical Imaging
    • Optical Coherence Tomography

    Background:

    • Optical Coherence Tomography (OCT) is a powerful imaging modality for cross-sectional visualization of biological tissues.
    • Simultaneous acquisition of structural, birefringence, and blood flow information can enhance diagnostic capabilities.
    • Existing OCT systems often require specialized hardware or complex processing for multi-functional imaging.

    Purpose of the Study:

    • To develop and demonstrate a multi-functional optical coherence tomography (MF-OCT) system capable of real-time acquisition, processing, and display.
    • To integrate the imaging of tissue structure, birefringence, and blood flow within a single, efficient system.
    • To validate the system's performance using data from human subjects.

    Main Methods:

    • Implementation of an efficient data processing pipeline for phase-resolved interference patterns.
    • Utilizing a high-speed fiber-based OCT system without dedicated hardware or extensive modifications.
    • Achieving real-time image acquisition at 2048 depth scans per second over a 5 mm x 1.2 mm area.
    • Real-time display with image updates at 32 frames per second in a rolling manner.

    Main Results:

    • Successful real-time acquisition, processing, and display of tissue structure, birefringence, and blood flow.
    • Demonstration of high-speed imaging capabilities with rapid data processing.
    • Visualization of microvasculature and tissue properties in the proximal nail fold of a human volunteer.

    Conclusions:

    • The developed MF-OCT system enables efficient, multi-functional imaging in real-time.
    • The system's performance is achieved through optimized data processing, minimizing hardware requirements.
    • This technology holds potential for advanced diagnostic and research applications in biomedical imaging.